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KMID : 0380620140460010115
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Korean Journal of Food Science and Technology
2014 Volume.46 No. 1 p.115 ~ p.120
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The Use of the Pathogen-specific Bacteriophage BCP8-2 to Develop a Rice Straw-derived Bacillus cereus-free Starter Culture
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Nadeeka Ban-da-ra
Chung Seo-Jin
Jeong Do-Yeon
Kim Kwang-Pyo
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Abstract
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The purpose of this study was to develop a rice straw-derived Bacillus cereus (B. cereus)-free starter culturefor traditional soybean fermented products using a B. cereus-specific bacteriophage, BCP8-2. To determine the optimalmedium that supports the growth of rice straw-derived microorganisms and BCP8-2 activity, 5 different culture media weretested. The 5% ground bean (GB) medium was selected for further study. No B. cereus was detected in the BCP8-2-treatedrice straw in GB medium, whereas B. cereus at a level of 107 CFU/mL was recovered in the no-phage control. The totalbacterial count reached approximately 109 CFU/mL regardless of phage addition. When the 16S rRNA sequence-basedmicrobial community was monitored using denaturing gradient gel electrophoresis (DGGE) and pyrosequencing, a similarmicrobial community was observed in the phage-treated and control samples. In conclusion, we demonstrate that phagecan be used to prepare a rice straw-derived B. cereus-free starter culture with minimal effect on natural microflora.
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KEYWORD
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Bacillus cereus, bacteriophage, rice straw, fermented food, starter culture
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